Fluorescent Polymer Microspheres
Fluorescent Polymer Microspheres emit bright and distinctive colours when illuminated by light of wavelengths shorter than the emission wavelength. This property improves their contrast and visibility relative to background materials.
Besides the features of conventional microspheres, these Fluorescent Microspheres offer improved sensitivity and detectability for analytical methods, yielding very low detection limits for both particle diameters and particle concentrations.
Applied Microspheres’ unique technology provides an exceptionally low fluorescence coefficient of variation (C.V.), making these Polymer Microspheres suitable as Fluorescent Reference Standards for laboratory research, instrument calibration and analytical applications.
Customisation, Stability and Available Formats
This product line was developed for general laboratory use. Applied Microspheres offers Fluorescent Polymer Microspheres in a wide variety of particle diameters that can be custom dyed with different fluorescent colours and intensity levels as required.
The dyes are incorporated during the polymerisation process, preventing dye leakage and ensuring long-term fluorescence stability.
The microspheres can be dispersed in both aqueous media and air without degradation of their fluorescent properties. They are available as aqueous suspensions or as dry powder. The aqueous suspension medium is specially formulated to prevent the formation of agglomerates, provide long-term stability and allow convenient storage at room temperature.
Where required, the polymer microsphere matrix can be modified to improve stability in specific solvents. Targeted colloidal and spectral adjustments also enable the particles to mimic complex biological components, including bacteria, making them suitable for advanced analytical studies, life science research and method development.
Detection Systems and Analytical Applications
Fluorescent Polymer Microspheres can be detected using flow cytometers, epifluorescence microscopes, confocal microscopes, fluorescence spectrophotometers and other fluorescence detection systems.
They can also be identified using mineral light or black light and may be observed directly in the sample matrix or collected on membrane filters for examination using fluorescence microscopy.
Their optical properties make them suitable as Confocal Microscopy Standards, Fluorescent Reference Standards and reference materials for fluorescence-based analytical techniques.
Applications and Usage
| ✔ Fluorescence microscopy | ✔ Detection using flow cytometers |
| ✔ Fluorescent instrument monitoring | ✔ Detection using epifluorescence and confocal microscopes |
| ✔ Confocal microscopy reference particles | ✔ Fluorescence spectrophotometry applications |
| ✔ Filtration media and systems testing | ✔ Flow cytometry instrument calibration and set-up |
| ✔ Vial and container cleaning studies | ✔ Analytical studies requiring high sensitivity and low detection limits |
| ✔ Flow tracing and fluid mechanics | ✔ Quality control and validation studies |
| ✔ Centrifugation and sedimentation studies | ✔ Biomedical and life science research |
| Size | Format | Quantity | Solids |
|---|---|---|---|
| 0.1 µm | Suspension | 3 mL / 50 mL | 1% |
| 0.5 µm | Suspension | 3 mL / 50 mL | 1% |
| 1 µm | Suspension | 3 mL / 50 mL | 1% |
| 5 µm | Powder / Suspension | 1 g / 3 mL / 50 mL | Dry / 1% |
| 10 µm | Powder / Suspension | 1 g / 3 mL / 50 mL | Dry / 1% |
| 15 µm | Powder / Suspension | 1 g / 3 mL / 50 mL | Dry / 1% |
| 20 µm | Powder / Suspension | 1 g / 3 mL / 50 mL | Dry / 1% |
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Frequently Asked Questions
What are Fluorescent Polymer Microspheres?
They are Fluorescent Microspheres with embedded dyes that provide stable fluorescence for calibration, imaging and analytical applications.
What are Fluorescent Polymer Microspheres?
They are Fluorescent Microspheres with embedded dyes that provide stable fluorescence for calibration, imaging and analytical applications.
What are Fluorescent Polymer Microspheres used for?
They are used in flow cytometry, fluorescence microscopy, Confocal Microscopy Standards, filtration studies and instrument calibration.
Why are embedded fluorescent dyes important?
Embedding dyes within the polymer matrix prevents dye leakage and ensures long-term fluorescence stability.
Can these microspheres be used as Fluorescent Reference Standards?
Yes. They are widely used as Fluorescent Reference Standards for instrument validation and analytical testing.
Are Fluorescent Polymer Microspheres available as suspensions and powders?
Yes. They are supplied as aqueous suspensions or dry powders without compromising fluorescent performance.
Which industries use Fluorescent Polymer Microspheres?
Life sciences, biotechnology, pharmaceuticals, research laboratories and analytical testing facilities.